Select the Vertical tab to load vertical scale lines. Click "Open" to load the selected images.
Click on "Load Image" to display the Open Image files dialog box from which horizontal lines may be selected for calibration. Select the Horizontal tab to load horizontal scale lines. Select the "Calibrate with Scale Line" tab to display the corresponding panel.
To calibrate with a scale line, select the Calibration Wizard command - under Measure - to display the Calibration Wizard window. Click "Calibration" to complete the calibration process.Ĭalibrate with Scale Line Using Motic Software Confirm the objective lens used to capture the selected image and then input the actual length of "OA" in the "Width" bar and the actual length of "OB" in the "Height" bar. The magnifier window under the image window will help you accurately place the points. Next, place a point (set as "A") that is horizontal with the center of the circle on the horizontal line, and place a point (set as "B") that is vertical with the center of the circle on the vertical line. (You can modify its color by clicking the Circle Color button to display the color palette.) Place the center of the circle (set as "O") in the middle of the scale cross. When an image is loaded, a circle will appear on it. Click on "Load Image" to display the Open Image files dialog box from which scale crosses may be selected for calibration. Select the "Calibrate with Scale Cross" tab to display the corresponding panel. To calibrate with a scale cross, select the Calibration Wizard command - under Measure - to display the Calibration Wizard window. When calibration is complete, click "Close" to close the Calibration Wizard window.Ĭalibrate with Scale Cross Using Motic Software. The Save Sign dialog box will display after you've clicked "Calibration." Click "Save" in the dialog box to save the calibration results and use them for measuring.(Note: Use the appropriate calibration circle slide and magnification suited to your microscope.) Click "Calibration" to complete the calibration process. Confirm the objective lens used to capture the selected image, then input the diameter of the calibration circle.Select "Load Image" to display the Open Image files dialog box from which calibration circles may be selected for calibration. Click the "Calibrate with Calibration Circle" tab to display the corresponding panel. To calibrate with a calibration circle, select the Calibration Wizard command - under Measure - to display the Calibration Wizard window.
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Watch this video to see how to calibrate your microscope using Motic Images Plus software.Ĭalibrate with Calibration Circle Using Motic Software